BIPHASIC MEDICAL ELECTROPORATOR

This instrument is a novel bio-medical electroporator for efficient, easy and rapid delivery/ insertion of bio-molecules, drugs into in vitro and animal experimental systems for drug delivery, transfection and gene therapy. 

Electroporation is commonly employed bio-medical technique for application in the field of drug delivery, gene therapy, cell fusion and sterilization of food and water. The technology is very much useful in efficient killing of tumor cells, which are otherwise resistant against treatment with radiation and drug with high potential to be applied in cancer treatment alone or in combination with radiotherapy and chemotherapy.

PRINCIPLE

The instrument operates by inducing Cell Electroporation, which is the process of formation of transient micropores in cellular plasma membrane by application of high intensity, short duration electric field. Application of voltage beyond threshold causes polarization of membrane components resulting in membrane permeabilization for exogenous molecules like drugs and DNA. The induced membrane permeability is reversible, provided the magnitude of the generated electric field do not exceed critical limit, beyond which the cell gets irreversibly damaged. Induced pores can remain open from seconds to minutes depending on the type of cell and electric pulse. With recent advancement in circuit and design of the instrument, the technology is rapidly growing for clinical applications of cancer and delivery of genes for various diseases.

SPECIFICATIONS

APPLICATION

Cell Treatment Chamber Solid Tumor Electrode

• Delivery of anti-tumor and anti- fungal drugs for cancer and trans-dermal skin diseases therapy.

• Transfection of bacterial cells by DNA.

• Gene therapy of human normal and tumor cells.

• Electro-insertion of proteins /enzymes.
  

INFRASTRUCTURE REQUIRED

• Testing equipments
  Digital Multi-meter, Digital Oscilloscope with probe 1: 10 
• Tools
  Pliers, Cutter, Soldering Iron solder wire, Sleeve remover. 
  Disordering pump, Drilling machine drill bits (1-10 mm), Screw driver, screws and nuts
• Mechanical and Electronics Assembling facility
• Components
  PVC wires components, Resistor capacitors, ICs, Transformers, Cabinet 
• Man Power
  One Electronics Diploma holder having 1-2 years experience in wiring electronic circuits, one Technician (12th Standard) having experience in mechanical jobs and one Helper. 
• Working area (approx.)
  Total                    :    250 Sq. ft. 
  Laboratory size  :   10 x 10 ft.
  Office size           :   10x 15 ft.