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Success Stories - Agriculture

Bhabha Atomic Research Centre, Mumbai, India has a broad based research programme in Food and Agriculture involving genetic improvement of crops through mutation breeding and biotechnological approaches. The other applications of radiation technology in agriculture are studies on fertilizer use efficiency, control of insect pests, monitoring of pesticide residues and preservation of agricultural produce.

Genetic Improvement of crop plants using induced mutations and crop breeding

Mutation breeding programmes to induce variability and use them in crop improvement began in the early sixties and a large spectrum of variability has been isolated and maintained in the following crops (See box below). The major emphasis is on oilseeds and pulse crops for higher productivity, resistance to biotic/abiotic stress and quality improvement.

Oilseeds

Pulses

Cereals

Other crops

Groundnut

Pigeonpea

Rice

Sesbania(green manure)
Jatropha
Bio Diesel

Mustard

Mungbean

Wheat

 

Sunflower

Uridbean

 

 

Linseed

Cowpea

 

 

Soybean

 

 

 

  Plant height and leaf size mutants in groundnut
Seed coat variability in cow pea
Extreme dwarf mutant in sunflower

 

 

Stripe Seed coat and Black seed coat sunflower

Brown seed coat and; yellow seed coat in mustard 

Direct mutants and their use in breeding programme have successfully resulted in the development of 26 high yielding varieties with quality traits.

Trombay crop varieties released and notified for commercial cultivation

Name

Year of Release

M: Maturity (days)

Y: Yield (kg/ha)

YI: Yield increase (%)

Released for

Remarks

Blackgram

TAU-1

1985       

M: 73

Y: 900

YI: 24

Maharashtra

Large seed,  Most popular variety in Maharashtra

TAU-2

1992

 M:73

Y: 950

YI:18

Maharashtra

High yielding

TPU-4

1992

M: 72

Y: 950

YI: 22

Maharashtra

Madhya Pradesh

Large seed

TU94-2

1999

M: 73

Y: 950

YI: 19-37

Andhra Pradesh Karnataka

Kerala,

Tamil Nadu

Resistant to Yellow Mosaic Virus

Greengram

TAP-7

 

1983

 

M: 64

Y: 750

YI: 23

 

Maharashtra Karnataka

 

Tolerant to powdery mildew

TARM-2

1994

M: Rabi: 80

Y: 1003

YI: 80

Maharashtra

Resistant to powdery mildew

TARM-1

1996

M: 78

Y: 1204

YI: 45

Maharashtra Gujarat, MP, AP, Kerala Karnataka, Tamil Nadu,

Orissa

Resistant to powdery mildew

TARM-18

1996

M: 65

Y: 1051

Maharashtra

Resistant to powdery mildew

TMB-37

2005

M: 63

Y: 1100

YI: 20

Eastern UP, Bihar, Jharkhand, Assam and West Bengal

Early maturity

Tolerant to yellow mosaic virus

Pigeonpea

TT-6

 

1983

 

M: 136

Y: 1275

YI: 15

 

MP,

Maharashtra

Gujarat, AP,

Karnataka,

Kerala

 

Large seed

TAT-10

1985

M: 112

Y: 950

Maharashtra

Early maturing

Soybean

TAMS-38

 

2005

 

M: 95

Y: 2318

YI: 20

 

Maharashtra

 

Early maturing, resistant to bacterial pustule, Myrothecium leaf spot

Groundnut

TG-1

 

1973

 

M: 130-135

Y: 2400-2500

YI: 15-20

 

Maharashtra, Gujarat

 

Large seed

TG-17

1985

M: 115-120

Y: 1700-2000

YI: 15-20

Maharashtra

Less branches

TG-3

1987

M: 110

Y: 2000-2500

Kerala

More branches

Somnath

(TGS-1)

1989

M: 110-125

Y: Kharif 2000

YI: 23

Gujarat

Large seed

Spreading habit

TAG-24

1991

M: Kharif 100-105 Summer 112-117

Y: kharif 1300

Summer 2500

YI: Kharif 24

Summer 50

Maharashtra

West Bengal

Rajasthan

Karnataka

Most popular in all ground nut growing state, identified as national variety, High yield potential (9000-10000kg/ha)

Semi dwarf habit, early maturity, high harvest index, high partitioning efficiency, wider adaptability

TG-22

1992

M: Kharif 115-120

Y: Kharif 1677

YI: 30

Bihar

Medium-large seed, fresh seed dormancy

TKG-19A

1994

M: 120-125

Y: summer 2000-2500

YI: 12-13

Maharashtra

Large seed, fresh seed dormancy

TG-26

1995

M: 110-120

Y: summer 2500

YI: 23-39

Gujarat Maharashtra, MP

Semi-dwarf, early maturity, high harvest index, high partitioning efficiency, fresh seed dormancy

Second popular TG variety, high yielding ability (9000-10000kg/ha), wider adaptability

TPG-41

2004

M: 120

Y: Summer 2407

YI: 26

All India

Large seed (65g 100 seeds) Fresh seed dormancy

On farm trials 4551kg/ha

49% increase

TG-37A

2004

M: 110

Y: Kharif 1993

YI: 26-38

Rajasthan, UP,

Gujarat , NE states, Punjab, Haryana

Fresh seed dormancy

Oil 51%

Mustard

TM-2

 

1987

 

M: 90

Y: 1370

YI: 25

 

Assam

 

Appressed pod

TM-4

1987

M: 95

Y: 1470

YI: 35

Assam

Yellow seed

Rice

Hari

 

1988

 

M: 135-140

Y: 6000

YI: 20

 

Andhra Pradesh

 

Slender grain type

Jute

TKJ-40

 

1983

 

M: 125-130

Y: 2800-3100

YI: 10-13

 

Orissa

 

High yielding

 

Some of the popular and recently released varieties are given below.

           

TPG 41 - new variety released for all India. Large      TG-37 A is released for Kharif/rabi summer season 
seed, 120 d maturity, 20 d dormancy, high oleate       with wider adaptability & seed dormancy of 15 days


         

TAMS 38: Early maturity (95 days)                             TMB- 37 (Trombay Mungbean): high yielding potential
Resistant:                                                                early maturity and tolerance to yellow virus disease
Pod shattering : (13 days after maturity)
Diseases: Bacterial pustule, Myrothecium leaf spot insects (moderate); stemfly, Girdle beetle.

The popular groundnut varieties are TAG-24 and TG-26. TAG24 has been identified as national check. In pulse crops, blackgram variety TAU-1 is the most popular in Maharashtra and maximum area is under cultivation of this variety.

                  TAG – 24 , TAU - 1 and TG – 26 
The entries with new traits that are being used as donors in breeding programme, include

1. TM-99-50: Resistant to powdery mildew and yellow mosaic virus diseases

2. TU – 88 -5 Resistant to yellow mosaic virus disease.

     

                                    Mungbean                                         Blackgram

Novel mutations which could be used in understanding the basic mechanism have been registered with National Bureau of Plant Genetic Resources (NBPGR), New Delhi, India

          

Ground nut leaf mutants, Small Leaf               Fasciation mutant in sunflower
 TG-18 AM                  

BARC has developed linkages with agricultural universities, seed corporations and progressive farmers to assure quality seeds to farmers. The Trombay crop varieties have been popularized through various awareness programmes, exhibitions, kisan melas and farmer’s rallies.

          

Seed multiplication of groundnut - TAG-24   and  mungbean - TMB-37

          

          Seed production plots               Trombay groundnut ready to reach the
            farmer

          

                                          Farmer's rally

Integrated Pest Management

The red palm weevil is an important pest of coconut palm. A new “T” olfactometer was designed and fabricated for carrying out response of red palm weevil pheromone to commercially available pheromone studies and to BARC synthesized ones.

Application of Sterile Insect Technique (SIT) to control red palm weevil in coconut

The other research programmes based on radioisotopes include,

  • Studies On Fertilizer Use Efficiency And Micronutrient Uptake In Plants
  • Monitoring Of Pesticide Residues

 Plant Molecular Markers

DNA based molecular characterization of BARC mutants was carried out using RAPD and ISSR markers.

Rice blast fungus identification

        

 Molecular characterization of radiation induced mutants of blackgram

             

 Plant Tissue Culture

Micropropagation protocols have been developed for banana, sugarcane, pineapple, potato, turmeric and ginger. Protocols for direct and indirect somatic embryogenesis have been established in banana, grapes and sugarcane.

Technology for the tissue culture production of banana has been transferred to MSSC, Akola, Maharashtra and Krishi Vigyan Kendra, Govt. of Pondicherry, Pondicherry. The promising clones of sugarcane derived from tissue culture are being evaluated at Marathwada Agricultural University Parbhani.

Tissue cultured plants established at BARC  

Micropropagation protocols have been developed for banana, sugarcane, pineapple, potato, turmeric and ginger. Protocols for direct and indirect somatic embryogenesis have been established in banana, grapes and sugarcane. 
Technology for the tissue culture production of banana has been transferred to MSSC, Akola, Maharashtra and Krishi Vigyan Kendra, Govt. of Pondicherry, Pondicherry. The promising clones of sugarcane derived from tissue culture are being evaluated at Marathwada Agricultural University Parbhani.

  Genetic transformation of plants

Transgenic groundnut, potato, mothbean and banana have been developed with marker genes and useful genes for disease/pest resistance and edible vaccines using Agrobacterium mediated and other gene transfer methods.

Crop
Gene
Trait
Tobacco; Banana Magainin Fungal resistance
Tobacco; potato Coat protein gene Potato virus ‘y’ resistance
Chickpea cry1ac gene Insect resistance
Tobacco; Banana Hepatitis b surface antigen coding ‘s’ gene Edible Vaccine

A protocol for the preparation of juice and powder from ripe banana has been developed

        

      

Environment & Bioremediation
Bioremediation organics, radionucliedes and heavy metals using microbes and plants are being studied. Biosensor for environmental monitoring is being studied.

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Biogas from kitchen waste

Biogas plant named “nisarguna” developed to process kitchen waste has been working satisfactorily. Digested slurry has good fertilizer value for crops. The biogas plant was able to process other forms of organic wastes like presoaked grass and shredded paper.

One biogas plant each of 1 tonne capacity and 5 tonne capacity were constructed at Hiranandani Estate, Thane, Maharashtra and INS Chilka, Orissa respectively .

FOOD PRESERVATION BY RADIATION PROCESSING - NEED TO PRESERVE FOOD

Radiation processing of food involves exposure of food to short wave electromagnetic radiations to achieve sprout inhibition, delay in ripening and senescence, insect disinfestation and elimination of food borne microbes, pathogens and parasites.

Food is

* vital for human existence.

* Conservation and preservation of food is a prerequisite for food security and

* it provides economic stability & self-reliance

In comparison with heat or chemical treatment, radiation processing is more effective and appropriate technology for preserving food.

SAFETY AND NUTRITIONAL ADEQUACY OF RADIATION PROCESSED FOODS

In India, the Ministry of Health and Family Welfare, amended the Prevention of Food Adulteration (PFA) Act Rules (1954) through a Gazette notification dated August 9, 1994, permitting radiation processing of onion, potato and spices for internal marketing and consumption. In 1998 and 2001 additional food items were permitted for radiation processing. The list of items approved for radiation processing under PFA Act Rules is given in Table-1.

Table-1:Food items approved for radiation preservation by under PFA Act Rules

Name of food

Purpose

Minimum Dose (kGy)

Maximum Dose (kGy)

Onion 

Potato 

Ginger

Garlic

Shallots (Small onion)

Sprout inhibition

0.03

0.06

0.03

0.03

0.03

0.09

0.15

0.15

0.15

0.15

Mango

Disinfestation  (Quarantine)

0.25

0.75

Mango

 

Semolina (sooji, rawa), Wheat atta and maida 

 

Raisin, figs and dried dates

Disinfestation  (Quarantine)

0.25

 

0.25

 

0.25

0.75

 

1.0

 

0.75

Meat and meat products including chicken

Shelf-life extension and pathogen control

2.5

4

Spices

Microbial decontamination

6

14

Pulses 

Disinfestation

0.25 

1.00 

Dried sea-foods

Disinfestation

0.25 

1.00 

Fresh sea-foods

Shelf-life extension

1.00 

3.00 

Frozen sea-foods

Pathogen control

4.00 

6.00 

 
Applications of radiation can be classified into

* Low Dose Applications

Sprout inhibition in bulbs and tubers

0.03 - 0.15 kGy

Delay in ripening and senescence

0.25 - 0.75 kGy

Insect disinfestation including quarantine treatment and elimination of food borne parasites

0.25 - 1 kGy

  * Medium Dose Applications  

Reduction of spoilage microbes to improve shelf- life of meat, poultry and seafoods under refrigeration

1.5 - 3 kGy

Elimination of pathogenic microbes in fresh and frozen meat, poultry and seafoods

3 - 7  kGy

Reducing number of microorganisms in spices to improve hygienic quality

10 kGy

* High Dose Applications

Sterilization of packaged meat, poultry and their products which are shelf-stable without refrigeration

25 - 70 kGy

Sterilization of hospital diets

25 - 70 kGy

French Beans

Radiation processing extended the shelf life of pre-packed whole as well as cut French beans by 12 days at 10 ºC. Similarly, encouraging results were obtained with pre-packed cut ash gourd. A combination treatment of hot water dip and radiation was found to extend the shelf-life of papaya by 12 days without affecting the quality with no significant differences in the vitamin content.

Wholesomeness of irradiated foods

No other method of food processing has been subjected to such a thorough assessment of safety as the radiation processing. The various aspects of wholesomeness and safety of radiation- processed foods have been studied in great detail. Irradiated or radiation processed foods are those that have been exposed to radiation to bring about the desired effect in food. Radioactive foods, on the other hand, are those that become contaminated with radionuclides, for example during a radioactive fall out as exemplified by Chernobyl butter. This type of contamination never occurs during radiation processing of food. Irradiation produces very little chemical changes in food. None of the chemical changes found to occur in food have been found to be harmful. Highly sensitive scientific tests carried out during the past 40 years have failed to detect any harmful chemical products in radiation processed foods. In fact, the chemical differences between radiation processed foods and non-irradiated foods are too small to be detected easily. In comparison to other food processing and preservation methods the nutritional value is least affected by irradiation. In fact, astronauts and cosmonauts have been taking radiation processed food as part of their ration on the various space flights from Apollo to space shuttle.

As in many other food processing procedures only food of good hygienic quality should be irradiated. It is very important that foods intended for processing are of good quality and handled and prepared according to good manufacturing practice (GMP) established by national and international standards . Like any other food treatment irradiation cannot reverse the spoilage process and make bad food good. A food that looks, smells and tastes bad, cannot be saved by any treatment including irradiation.

CONSUMER RESPONSES

A number of surveys have been conducted nationally and internationally to study the response of consumers to radiation processed food. To evaluate the public perception on radiation processed foods in India, a questionnaire was prepared. Response to tasting of irradiated foods conducted in Mumbai at several seminars organized by professional bodies, and seminars for specific target groups like University Students, University and School teachers, officers and staff of Army Supply Depot, Mumbai and consumer and food industry groups have shown overwhelming positive response to eating snack items made from irradiated onions, potatoes and spices. The Defence Research and Development Organization (DRDO) have conducted `Ahar Utsavs' with irradiated foods among Defence personnel at several locations in the country with great success. In view of the advantages of radiation processing technology for spices, Annapurna Mahila Mandal, a women’s cooperative, launched the sale of radiation processed spices under the trade name ‘Purnanna’ at their outlet SHRADDHA. The radiation processed spice kept for sale at the counter by Annapurna Mahila Mandal, had good consumer response.

International Approval

In 1980 a joint FAO/IAEA/WHO Expert Committee on Food Irradiation (JECFI) reviewed the extensive data on wholesomeness collected up to that time and concluded that irradiation of any commodity up to an over all dose of 10 kGy presents no toxicological hazards and introduces no special nutritional or microbiological problems. An Expert Group constituted by WHO in 1994 once again reviewed the wholesomeness data available till then and validated the earlier conclusion of JECFI. In 1998 another Expert Group constituted by WHO/FAO/IAEA affirmed the safety of food irradiated to doses above 10 kGy. In view of this recommendation the Codex Committee on Food Standards of The Codex Alimentarius Commission has also revised in 2003 the Codex General Standard for Irradiated Foods that sets standards for processed foods. In addition, a number of scientific bodies and associations have also endorsed the safety of radiation processed foods. These include, to name a few:

  • American Medical Association
  • The American Gastro-entrological Association
  • American Dietetics Association
  • American Meat Institute
  • Institute of Food Technologists

The Agreements on Sanitary and Phytosnitary (SPS) Practices and Technical Barriers to Trade (TBT) under the World Trade Organization (WTO) has provided a distinct incentive to the adoption of irradiation as an SPS measure in international trade under the principle of equivalence. Thus, irradiation can be applied to overcome quarantine barriers, and to hygienize products for international trade.

Commercial prospects

The estimated cost of setting up a commercial radiation processing facility is in the range of Rs.5-7 crores.. Many variables affect food costs, and one of them is cost of processing. Any processing will add to the cost of food. But processing also brings many hidden benefits to consumers in terms of increased availability, storage life, distribution, and improved hygiene of food. It can have a stabilizing effect on market price of commodities by reducing storage losses resulting in increased availability of produce. These factors should therefore be taken in to account while considering the economics. Generally speaking, irradiation costs may range from Rs.0.25 to 0.50 / kg for a low dose application such as sprout inhibition of potato and onion and insect disinfestation in cereals and pulses to Rs.1-3/kg for high dose applications such as treatment of spices for microbial decontamination. The costs could be brought down in a multipurpose facility treating a variety of products round the year.

  • India has one of the world’s largest domestic markets. Huge quantities of cereals, pulses, their products, fruits and vegetables, poultry meat, sea-foods, and spices are procured, stored, and distributed throughout the length and breadth of the country. For domestic consumption radiation processing can be used to facilitate storage, movement and distribution of these commodities from production centers to consumption centers and to prevent post harvest losses during these operations. Thus, potential for harnessing radiation technology for the benefit of food industry is really immense.

Technology Demonstration Units

The Department of Atomic Energy has set up two technology demonstration units in India. The Radiation Processing Plant at Vashi, Navi Mumbai, is a 30- ton per day capacity unit capable of delivering medium and high doses. The plant is being operated by the Board of Radiation & Isotope Technology (BRIT) since January 2000. Another unit KRUSHAK (Krushi Utpadan Sanrakshan Kendra) at Lasalgaon near Nashik, is primarily a low dose irradiator capable of delivering doses less than 1 kGy to agricultural commodities. The unit is a 10- ton per hour capacity plant built and operated by BARC. Already, more than a dozen MoUs have been signed with BARC/BRIT by the entrepreneurs interested in setting up radiation processing units.

KNOW-HOW AND TECHNOLOGY TRANSFER Expertise and know-how for designing, fabrication and commissioning of irradiators is available in the country with the Department of Atomic Energy.

 
 
   
     

 
 
   
 
 
   
 
 
   
 
 
   
 
 
   
 
 
   
 
 
   
 
 
   
 
 
   
 
 
   
 
 
   
 
 
   
 
 
   
 
 
   
 
 
   
 
 
   
 
 
   
     

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